REINFORCED CLOSTRIDIAL AGAR 500g

Description
Reinforced Clostridial Agar is a solid version of Oxoid Reinforced Clostridial Medium CM0149, suitable for the cultivation and enumeration of clostridia and other anaerobes, lactobacilli, and many other species of bacteria. Reinforced Clostridial Agar does not differ significantly in performance from the pork-starch-pea agar of Anderson¹ for the count of anaerobes. It is employed for the estimation of clostridia in food - see below (also Barnes et al.² and Angelotti³). Attenborough and Scarr⁴ employed RCM Agar, in conjunction with Membrane Filters, for the count of Clostridium thermosaccharolyticum in sugar.

Reinforced Clostridial Agar is also frequently employed for the investigation of intestinal flora: Perry et al.⁵ for investigation of bovine rumen streptococci; Williams Smith & Crabb⁶ used the Oxoid medium with added sodium chloride or blood for counts on human or animal faeces; Barnes & Goldberg⁷ employed the Oxoid medium with added chlortetracycline hydrochloride or sodium azide and ethyl violet, for the examination of poultry faeces; the Oxoid medium was also used by Goldberg et al.⁸ for the examination of poultry faecal samples. Williams Smith⁹ employed Oxoid Reinforced Clostridial Agar with added blood for the `total’ and Lactobacillus count of human and animal faeces; with added blood and neomycin for determination of Bacteroides, anaerobic Gram-negative cocci, `total’ streptococci, sporeformers, yeasts, and `actino’ types.

Sneath¹⁰ used Oxoid RCM Agar and other media for the anaerobic count of micro-organisms from soil samples up to approximately 300 years old. Gregory et al.¹¹ also employed the Oxoid medium for the estimation of anaerobes in moulding hay.

Specification